GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.
GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.
GenScript's Monofinity A Resin is made of recombinant protein A as ligand, which not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also enhances chemical stability under Cleaning-in-place (CIP) conditions.